Analysis of using controlled slow cooling

Ovarian tissue cryopreservation as fertility preservation options for cancer patients. Process as defined in claim 11, wherein the dumped slag is left to cool for about 2 hours after dumping. Process as defined in claim 8, wherein the slag is poured in a ton ladle and allowed to cool for about 24 hrs until its center has substantially solidified.

All oocytes became translucent after freezing which complicated the use of GVBD test in vitro maturation of oocytes followed by observation of germinal vesicle breakdown which results in oocytes becoming translucent.

Results also showed that the ATP level in oocytes decreased significantly immediately after thawing. Jaw Crusher then by a 7 ft. Abstract Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment.

On the other hand, the exact thawing temperature seems to have only minor effect on sperm viability, acrosomal status, ATP content, and DNA. Previously we reported some preliminary results on cryopreservation of zebrafish Danio rerio oocytes using controlled slow cooling and determined the optimum cryoprotective medium and cooling rate for stage III zebrafish oocytes.

In addition, water cooling has the advantage of enhancing the brittleness of the slag, while not substantially affecting the subsequent copper recovery.

The decision tree learning model. Treatment of sperm with heparin binding proteins prior to cryopreservation showed decreased cryoinjury and generation of ROS.

The improvement in milling characteristics is shown by the graph of fineness of grind versus tailings assay to slags cooled in beds and in ladles as illustrated in the drawing.

The numbers on each axis represent the categories identified within a decision variable. These alternatives are optimal in their own respect; however, overall, the employment of integrins is by far the best approach.

Tests on ladle-cooled slags produced by the continuous smelting and converting of copper concentrate to copper have also shown that improved tailings and concentrate grade can be obtained as compared to bed cooled slags as shown in the following Table II: Water is sprayed or otherwise added on top of the slag in the ladle while the slag is allowed to cool until it is substantially solidified.

However, there is a limit to the DTLA approach in that generalization cannot be delivered as there are no predictions outside the very local and very limited scope. We have used the technology of X-ray computed tomography to assess the concentration and distribution of dimethyl sulfoxide one of the cryoprotectants most used in fertility preservation inside pieces of bovine ovarian tissue after its cryopreservation.

One method of recovering this copper and other metals is slag milling followed by flotation.

A Bayesian approach to optimizing cryopreservation protocols

As such, the proper diagnostic is the variable R-squared at each branch as shown in Fig. It is therefore the object of the present invention to improve recovery of the non-ferrous metals in the slag by providing controlled cooling of non-ferrous slags so as to obtain particles of non-ferrous metals which are on the average larger in size than the ones obtained with the prior art coolling methods.

A summary scatter-plot analysis of key values for meta-data on main decision factors. Cryoprotectant media may be supplemented with either egg yolk or soy lecithin, with the two having no statistically significant differences compared to each other regarding motility, morphology, ability to bind to hyaluronate in vitro, or DNA integrity after thawing.A process for controlled slow cooling of non-ferrous smelting slags, such as copper slags, for the recovery of the non-ferrous metals contained therein by subsequent crushing, milling and flotation operations is disclosed.

The process comprises the steps of pouring the molten slag into a ladle, allowing the slag to slowly cool and solidify in the ladle at. These predictive syntheses demonstrate a generalized accuracy of 79% across the meta-data while the accuracy for predictive analytics for a specified case whereby samples are all cryopreserved in 3D natural RGD-containing matrices, using controlled slow-cooling with sucrose for lyoprotection, in a two-step loading process shows an 89% accuracy.

Semen cryopreservation

Semen cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm cells. Semen is frozen using either a controlled-rate, slow-cooling method (slow programmable freezing or SPF) or a newer flash-freezing process known as vitrification.

Cryopreservation of gametes provides a promising method to preserve fish genetic material. Previously we reported some preliminary results on cryopreservation of zebrafish (Danio rerio) oocytes using controlled slow cooling and determined the optimum cryoprotective medium and cooling rate for stage III zebrafish the present.

studies on cryopreservation of zebrafish (danio rerio) oocytes using controlled slow cooling and vitrification. However, the effectiveness of vitrification in relation to slow cooling with respect to pregnancy rates has so far not been evaluated by a randomised, controlled trial.

The aim of this study is to investigate whether vitrification significantly improves embryo survival and ongoing pregnancy rates when compared to embryos frozen by slow cooling.

Analysis of using controlled slow cooling
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